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目的检测基底/肌上皮标记物在三联阴性乳腺癌的表达情况,并分析基底细胞样癌的形态学特征。方法收集69例三联阴性乳腺癌存档蜡块,用单克隆抗体EGFR、CK5/6、CK14、CK17、SMA和CD10进行免疫组化MaxVisionTM法染色,并将其分为基底细胞样型(BLs)(表达任意一种标记物)和Null型(均阴性)。重点观察两者的形态学差异。结果BLs型48例(69.6%),Null型21例(30.4%)。具有统计学差异的形态学特征包括巢片状结构、推挤或部分推挤性边界、组织学分级高、核分级高和高核分裂指数,其中BLs型中42例呈巢片状结构(87.5%),26例呈推挤或部分推挤性边界(54.2%)。结论基底细胞样型与Null型乳腺癌具有明显的形态学差异,最具诊断价值的形态特点是巢片状结构、组织分级高、中到大量淋巴细胞浸润、推挤或部分推挤性边界和高核分裂指数。最具应用价值的标记物是CK5/6。
Objective To detect the expression of basal / myoepithelial markers in triple negative breast cancer and to analyze the morphological characteristics of basal cell carcinoma. Methods Totally 69 cases of triple negative breast cancer tissues were collected and analyzed by MaxVisionTM immunohistochemical staining with monoclonal antibodies EGFR, CK5 / 6, CK14, CK17, SMA and CD10, and divided into basal cell type (BLs) Expressing any of the markers) and Null type (all negative). Focus on the morphological differences between the two. Results BLs type 48 cases (69.6%), Null type 21 cases (30.4%). There were statistically significant morphological features including the lamellar structure, pushing or partial pushing border, high histological grade, high nuclear grade and high mitotic index, of which 42 cases of BLs type lamellar structure (87.5% ), 26 cases were pushed or partially pushed the border (54.2%). Conclusions There are obvious morphological differences between basal cell type and Null type breast cancer. The most diagnostic morphological features are nest-like structure, high histological grade, moderate to large lymphocytic infiltration, push or partial push border and High nuclear fission index. The most valuable marker is CK5 / 6.