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目的探讨调控纤维蛋白沉积水平对肾脏炎症反应的影响及其机制。方法实验分脂多糖诱导大鼠肾小球纤维蛋白沉积(LPS组);氨甲环酸增加纤维蛋白沉积(TL组);尿激酶减少纤维蛋白沉积(UT组)。肾组织单核细胞趋化蛋白-1(MCP-1)及血管内皮钙黏附蛋白(VE-Cad)mRNA和蛋白质表达分别用RT-PCR和免疫组织化学检测。结果(1)TL组与LPS,UT组纤维蛋白沉积、炎细胞数目差异有统计学意义(P<0.05);(2)TL组与LPS,UT组的MCP-1、VE-CadmRNA及蛋白质表达水平差异亦有统计学意义(P<0.05)。结论肾小球纤维蛋白沉积具有促炎症作用;纤维蛋白沉积的促炎症作用与其调节MCP-1、VE-Cad表达有关。
Objective To investigate the effect of fibrin deposition on renal inflammatory response and its mechanism. METHODS: Lipopolysaccharide was used to induce glomerular fibrin deposition (LPS group). Tranexamic acid increased fibrin deposition (TL group) and urokinase decreased fibrin deposition (UT group). The mRNA and protein expressions of MCP-1 and VE-Cadherin in renal tissue were detected by RT-PCR and immunohistochemistry respectively. Results (1) There was a significant difference in fibrin deposition and inflammatory cell count between TL group and LPS group and UT group (P <0.05). (2) MCP-1, VE-Cad mRNA and protein expression in TL group, LPS group and UT group The difference was also statistically significant (P <0.05). Conclusion Glomerular fibrin deposition has a proinflammatory effect. The proinflammatory effect of fibrin deposition is related to the regulation of MCP-1 and VE-Cad expression.