为探讨一氧化氮及抗氧化酶在石棉致病中的作用 ,用改良 Myrvik法收集兔肺泡巨噬细胞 (AM)进行体外培养 ,采用硝酸还原酶法测定 U ICC温石棉处理后兔 AM培养液中亚硝酸根 (NO2 - )的含量 (NO的静态氧化形式 ) ,同时利用黄嘌呤及黄嘌呤氧化酶反应系统测定 AM的细胞裂解液中 SOD的活性 ,和酶 -底物反应形成有色物质来测定 AM的细胞裂解液中 NOS和 GSH - Px的酶活性。结果显示 :随 UICC温石棉剂量的增加出现以下情况 :1 AM死亡率升高 ,细胞活性下降 ;2 AM培养液中的 NO- 2 含量增加 ,AM细胞裂解液中 SOD和 GSH - Px的活性降低 ,且以上均有剂量 -效应关系 (r1 =0 .6 1 1 4 ,P<0 .0 5 ;r2 =- 0 .5 1 2 6 ,P<0 .0 1 ;r3=- 0 .91 37,P<0 .0 1 ) ;3NOS在较低剂量组间呈剂量 -反应性增加 ,而在较高剂量组间呈剂量 -反应性降低 ,出现一个单峰曲线 ;4测得的NO的含量分别与 SOD和 GSH- Px活性呈负相关关系 (r1 =- 0 .71 2 5 ,P<0 .0 5 ;r2 =- 0 .8496 ,P<0 .0 5 ) ;5 NO和NOS在较低剂量组间呈正相关关系 ,在较高剂量组间呈负相关关系。以上提示 NO及其抗氧化酶在温石棉致病中具有一定作用 To investigate the role of nitric oxide and antioxidant enzymes in the pathogenesis of asbestos, rabbit alveolar macrophages (AM) were collected by modified Myrvik method and cultured in vitro. Nitric acid reductase (NO 2 -) (NO in the form of static oxidation). Meanwhile, the activity of SOD in the cell lysate of AM was measured by the xanthine and xanthine oxidase reaction system, and the reaction of the enzyme and the substrate to form a colored substance The enzymatic activities of NOS and GSH - Px in AM cell lysates were determined. The results showed that: with the increase of UICC chrysotile, the following conditions were observed: the mortality of 1 AM increased and the cell activity decreased; the content of NO - 2 in 2 AM medium increased and the activity of SOD and GSH - Px in AM cell lysate decreased , And all of them had dose-effect relationship (r1 = 0.61 1 1 4, P <0. 05; r2 = - 0 .5 1 2 6, P <0 1; r3 = -0.91 37 , P <0.01). Compared with the lower dose group, 3NOS showed a dose-response increase, while the dose-response decreased in the higher dose group, showing a unimodal curve. The NO content measured by 4 Respectively, and SOD and GSH-Px activities were negatively correlated (r1 = - 0.7125, P <0.05; r2 = - 0.88496, P <0.05) Low-dose group was a positive correlation between the higher dose group was negatively correlated. The above tips NO and its antioxidant enzymes in chrysotile pathogenesis has a role