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对佛手瓜下胚轴再生植株、试管苗移栽及大田定植进行了试验，结果表明：在ＭＳ＋ＩＡＡ０．１ｍｇ／Ｌ＋ＢＡ１．０ｍｇ／Ｌ＋ＬＨ２００ｍｇ／Ｌ培养基上容易形成下胚轴愈伤组织；愈伤组织分化芽的培养基为ＭＳ＋ＩＡＡ０．５ｍｇ／Ｌ＋ＢＡ０．５ｍｇ／Ｌ，诱导分化率可达５７５％；顶芽和带腋芽茎段生根的培养基分别为ＭＳ＋ＩＡＡ１．０ｍｇ／Ｌ和１２ＭＳ＋ＩＡＡ０．３ｍｇ／Ｌ，生根率分别为８１．８％和８３．３％；移栽培养土用土砂粪＝３２１的灭菌土，幼苗成活率达７５％；大田定值，平均单株结果８８．４３个，平均单果重２４０．２３ｇ．
The hypocotyls regenerated plantlets, transplanted in vitro plantlets and field colonies were tested. The results showed that hypocotyl callus could be easily formed on MS + IAA0.1mg / L + BA1.0mg / L + LH200mg / L medium; callus The medium of differentiation buds was MS + IAA0.5mg / L + BA0.5mg / L and the induction rate of differentiation was up to575%. The medium for MS and IAA1.0mg / L and12MS + IAA0.3mg / The rates were 81.8% and 83.3% respectively. The survival rate of seedlings was 75% for the transplanted culture soil with sterilized soil with dung = 321. The average single fruit weight was 88.43 and the average fruit weight 240.23g.