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目的:为验证肝纤维化动物模型和慢性肝病患者肝组织中,转化生长因子(TGF-β1)的mRNA表达增强。作者分析肝硬变患者外周血TGF-β1mRNA和血清中有活性的TGF-β1蛋白及其结果与型前胶原N端肽(PP)血清水平间的关系。方法:肝硬变患者50例,健康自愿者8人,取其外周血,分离单核细胞(PBMC),提取总RNA,用巢式RT-PCR扩增TGF-β1mRNA并测定血清中TGF-β1和PP的水平。结果:分析表明患者外周血单核细胞(PBMC)中TGF-β1mRNA有高阳性率的表达。血清中TGF-β1与PP的水平均明显高于对照。结论:研究结果证实肝硬变患者TGF-β1基因在外周血中表达增强,与PP血清水平相关,表明TGF-β1可能在肝纤维化、胶原合成中起重要作用
OBJECTIVE: To verify the expression of transforming growth factor (TGF-β1) mRNA in liver tissue of animal models of liver fibrosis and chronic liver disease. The authors analyzed the relationship between TGF-β1 mRNA in peripheral blood and serum TGF-β1 protein in cirrhotic patients and the serum levels of procollagen N-terminal peptide (PP). Methods: Fifty patients with cirrhosis and eight healthy volunteers were enrolled. Peripheral blood was collected and mononuclear cells (PBMCs) were isolated. Total RNA was extracted and the expression of TGF-β1 mRNA was detected by nested RT-PCR. And the level of PP. Results: Analysis showed that TGF-β1 mRNA in peripheral blood mononuclear cells (PBMCs) of patients had a high positive rate of expression. Serum TGF-β1 and PP levels were significantly higher than the control. CONCLUSIONS: The results confirm that the expression of TGF-β1 gene in peripheral blood of patients with cirrhosis is increased, which is correlated with the serum level of PP, indicating that TGF-β1 may play an important role in hepatic fibrosis and collagen synthesis