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Objective:To analyze the structure of aquaporins-3(AQP-3) from Schistosoma japonicum(SJAQP-3) using bioinformalical methods,and to provid of references for vaccine targets research.Methods:Protparam,BepiPred,TMHMM Server,MLRC,Geno3d,DNA star software packages were used to predict the physical and chemical properties,hydrophilicity plot, flexibility regions,antigenic index,surface probability plot,secondary structure,and tertiary structure of amino acid sequence of SJAQP-3.Results:SJAQP-3 had six transmembrane regions and two half-spanning helices that form a central channel.The half-spanning helices fold into the centre of the channel.Either of the half-spanning helix had a conserved motif of NPA common to all aquaporins.Predicted linear B-Cell epitopes were most likely at the N-terminal amino acid residues of Saa-7aa,59aa- 62aa,225aa-230aa,282aa -288aa,294aa -29Saa and 305aa -307aa area.59aa- 62aa,22Saa-230aa located outside the membrane,the others located inside the cell.Conclusions:SJAQP-3 is a integral membrane protein in Schistosoma japonicum tegument.There are six potential epitopes in SJ AQP-3.It might be a potential molecular target for the development of vaccines.
Objective: To analyze the structure of aquaporins-3 (AQP-3) from Schistosoma japonicum (SJAQP-3) using bioinformalical methods, and to provid of references for vaccine targets research. Methods: Protparam, BepiPred, TMHMM Server, MLRC, Geno3d, DNA star software packages were used to predict the physical and chemical properties, hydrophilic plot, flexibility regions, antigenic index, surface probability plot, secondary structure, and tertiary structure of amino acid sequence of SJAQP-3.Results: SJAQP-3 had six transmembrane regions and two half-spanning helices that form a central channel. The half-spanning helices fold into the center of the channel. Either of the half-spanning helix had a conserved motif of NPA common to all aquaporins. Predicted linear B-Cell epitopes were most likely at the N-terminal amino acid residues of Saa-7aa, 59a-62aa, 225aa-230aa, 282aa -288aa, 294aa -29Saa and 305aa-307aa area.59aa- 62aa, 22Saa-230aa located outside the membrane, the others located inside the cell.Conclusions: SJAQP-3 is an integral membrane protein in Schistosoma japonicum tegument. There are six potential epitopes in SJ AQP-3. It might be a potential molecular target for the development of vaccines.