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目的探讨HIV-1 B’亚型病毒感染者针对辅助蛋白Vpr的细胞免疫(CTL)反应特征及其与病毒复制控制的关系。方法利用检测IFN-γ分泌的ELISPOT方法,以覆盖HIV-1 B亚型Vpr蛋白全长的重叠肽段作为刺激抗原检测143例未接受抗病毒治疗的HIV-1 B’亚型病毒感染者针对Vpr蛋白的特异性细胞免疫反应,并分析其与病毒载量的关系。结果有16.8%的感染者可以产生针对Vpr蛋白的特异性CTL反应;能识别至少一条Vpr多肽的感染者的病毒载量低于不能识别Vpr多肽的感染者的病毒载量,差别有统计学意义(P=0.0191);对VPR-B-3多肽的识别与低病毒载量紧密相关,该多肽可能包含与Vpr蛋白的生物学功能相关的关键氨基酸位点;Vpr蛋白区多肽在人群中的识别水平的差异与其序列变异程度有关。结论Vpr蛋白特异性的CTL反应与宿主对病毒复制的控制具有一定的相关性,对Vpr蛋白区所包含的CTL表位进行鉴定并探讨其在感染过程中的作用,可为HIV疫苗设计提供参考依据。
Objective To investigate the cellular immune response (CTL) response to helper protein Vpr in HIV-1 B ’subtype virus and its relationship with virus replication control. Methods The ELISPOT method for detecting the secretion of IFN-γ was used to detect 143 HIV-1 B ’subtype virus-infected patients who did not receive antiviral therapy using overlapping peptides covering the full-length of HIV-1 B subtype Vpr protein as stimulating antigen Vpr protein-specific cellular immune response and analyze its relationship with viral load. As a result, 16.8% of the infected patients could produce specific CTL responses to Vpr protein. The viral load of those infected with at least one Vpr polypeptide was lower than that of the unprotected Vpr polypeptide. The difference was statistically significant (P = 0.0191). The identification of VPR-B-3 polypeptide is closely related to the low viral load. The polypeptide may contain key amino acid sites related to the biological function of Vpr protein. The recognition of Vpr protein in human population The level of variation is related to the degree of sequence variation. Conclusion Vpr protein-specific CTL response has some correlation with host control of viral replication. Identification of CTL epitopes contained in Vpr protein region and its role in the process of infection may provide a reference for the design of HIV vaccine in accordance with.