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对山西省农业科学院经济作物研究所保存的75份花生材料(包括28个已审定的花生品种和47个地方品种)进行了包括株型、茸毛的有无、叶色、粒形、叶形、生长习性、开花习性、粒大小、粒色等表型性状的Shannon-Weaver遗传多样性指数(简称SWI)和Simpson遗传多样性指数(简称SI)分析。结果表明:参试的75份花生品种遗传多样性指数分别为SWI=0.924,SI=0.500,其中以开花习性最低(SWI=0.139,SI=0.014),而Shannon-Weaver指数以粒色最高为1.841,Simpson指数为0.712。利用48对SSR引物对这些材料进行了遗传多样性分析,结果如下:(1)在48对花生的SSR引物中,有35对(占所用引物总数的72.9%)具有多态性,共检测到215条多态性条带,平均每对引物可扩增6条多态性带。(2)根据SSR扩增结果对75份材料的聚类分析结果表明,这些材料的相似系数(GS)为0.25~0.85,平均GS值为0.55。28个审定品种的相似系数为0.39~0.85,平均为0.60。
A total of 75 peanut materials (including 28 validated peanut varieties and 47 local varieties) preserved in the Institute of Economic Crops of Shanxi Academy of Agricultural Sciences were collected, including plant type, hairiness, leaf color, grain shape, leaf shape, Growth habits, flowering habits, grain size, grain color and other phenotypic traits Shannon-Weaver genetic diversity index (SWI) and Simpson genetic diversity index (referred to as SI) analysis. The results showed that the genetic diversity index of 75 peanut cultivars were SWI = 0.924 and SI = 0.500, respectively, among which the lowest flowering habit (SWI = 0.139, SI = 0.014) and Shannon-Weaver index , Simpson index is 0.712. 48 pairs of SSR primers were used to analyze the genetic diversity of these materials. The results were as follows: (1) Of the 48 pairs of SSR primers for peanut, 35 pairs (72.9% of the total primers used) were polymorphic and were detected 215 polymorphic bands, the average of each primer can amplify 6 polymorphic bands. (2) According to the results of SSR amplification, the results of cluster analysis on 75 materials showed that the similarity coefficient (GS) of these materials was 0.25 ~ 0.85, the average GS value was 0.55. The similarity coefficient of 8 certified cultivars was 0.39 ~ 0.85, The average is 0.60.