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淋巴囊肿病病毒感染牙鲆鳃细胞系FG-9307,提取细胞总RNA,采用RT-PCR法成功获得了淋巴囊肿病病毒主要衣壳蛋白0.6kb基因片段。构建其原核表达载体,IPTG诱导表达。结果表明,该融合蛋白分子量约48kD,可与抗LCDV多克隆血清特异反应。为LCDV基因工程疫苗的研制奠定了实验基础。
Lymphocystis disease virus infection Flounder gill cell line FG-9307, total cellular RNA was extracted, RT-PCR method was successfully obtained Lymphocystis disease virus capsid protein 0.6kb gene fragment. The prokaryotic expression vector was constructed and induced by IPTG. The results showed that the fusion protein molecular weight of about 48kD, anti-LCDV polyclonal serum specific reaction. It laid the experimental foundation for the development of LCDV genetic engineering vaccine.