AIM: To evaluate the effect of parenteral and enteral nutrition combined with octreotide on pancreatic exocrine secretion of the patients with pancreatic fistula.METHODS: Pancreatic juice, drained directly from the pancreatic fistula, was collected, and the volume, protein,amylase, HCO3-, K+, Na+ and Cl- were determined on d 1, 4and 7 before and after 7-d treatment with octreotide,respectively.RESULTS: No differences in exocrine pancreatic secretion were observed during the enteral and parenteral nutrition period (t = 2.03, P＞0.05); there were significant decreases in pancreatic juice secretion volume, protein, amylase,HCO3-, K+, Na+ and Cl- after parenteral and enteral nutrition combined with octreotide compared with octreotide pretreatment (t = 4.14, P＜0.05).CONCLUSION: There is no stimulatory effect on the pancreatic secretion by intrajejunal nutrition and parenteral nutrition. Octreotide is effective on the reduction of pancreatic fistula output.