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目的:通过检测血清TNF-α,IL-6的变化,评价乌司他丁对小肠缺血再灌注损伤的保护作用及机制。方法:健康Wistar大鼠84只,通过夹闭肠系膜上动脉(SMA)60min制作肠缺血模型,随机分成假手术组(C),肠缺血再灌注组(I),UTI治疗组(U)。根据缺血后再灌注时间不同又将I组和U组分成0min、2h和6h组。I组、U组于手术前经尾静脉分别注入生理盐水2mL、乌司他丁5×104U/kg,假手术组仅分离SMA,不夹闭血管。于各时点取腹主动脉血测定血清TNF-α、IL-6含量。结果:肠缺血再灌注各时相点均引起血清TNF-α、IL-6的变化,与假手术组相比,各时点TNF-α值显著升高(P<0.01),IL-6显著升高(P<0.01)。U组0min、2h血清TNF-α值低于相应时点的I组(P<0.01);U组0min、2h、6h血清IL-6值低于相应时点的I组(P<0.05)。结论:乌司他丁可减轻小肠缺血再灌注后的炎症反应。
Objective: To evaluate the protective effect and mechanism of ulinastatin on intestinal ischemia-reperfusion injury by detecting the changes of serum TNF-α and IL-6. Methods: A total of 84 healthy Wistar rats were randomly divided into three groups: sham-operation group (C), gut ischemia-reperfusion group (I) and UTI treatment group . Group I and group U were divided into 0min, 2h and 6h groups according to different reperfusion time after ischemia. In group I and group U, saline 2 mL and ulinastatin 5 × 104 U / kg were injected into caudal vein respectively before operation. Sham operation group only separated SMA and did not clamp blood vessel. Serum levels of TNF-α and IL-6 were measured by abdominal aorta blood at various time points. Results: The levels of TNF-α and IL-6 in sera caused by intestinal ischemia-reperfusion were significantly higher than those in sham-operation group at each time point (P <0.01) Significantly increased (P <0.01). The level of TNF-α in group U at 0 and 2 hours was lower than that in group I at the corresponding time point (P <0.01). The level of IL-6 in group U at 0, 2 and 6 hours was lower than that at group I (P <0.05). Conclusion: Ulinastatin can reduce inflammation after intestinal ischemia-reperfusion.