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以新世纪杏(Armeniaca L.cv.Xinshiji)为试材,采用RT-PCR方法,克隆了果实中苹果酸脱氢酶的编码基因,并对其在不同时期的表达进行了定量检测。序列分析表明:苹果酸脱氢酶基因的近全长cDNA长度为1081bp,它与Gen Bank中登记号为AF367442.1的桃子的苹果酸脱氢酶基因高度同源,核苷酸序列同源性为99%;该基因已在Gen Bank中注册,登记号为HQ189434。苹果酸脱氢酶基因的表达量在果实发育前期最高,随着果实的发育表达量逐渐降低,在果实发育后期表达量略有上升,其变化趋势与苹果酸脱氢酶的活性变化规律相一致,但基因的表达量较酶活性变化幅度小。
In the new century, Armeniaca L.cv.Xinshiji was used as experimental material, and the gene encoding malate dehydrogenase was cloned by RT-PCR and the expression of malate dehydrogenase was detected quantitatively at different stages. Sequence analysis showed that the full-length cDNA of malate dehydrogenase gene was 1081 bp in length, which was highly homologous to the malate dehydrogenase gene of peach with accession number AF367442.1 in GenBank. The nucleotide sequence homology Was 99%; this gene has been registered with Gen Bank under the accession number HQ189434. The expression level of malate dehydrogenase gene was the highest in early fruit development stage, and decreased gradually with the development of fruit. The expression level of malate dehydrogenase gene increased slightly in the late stage of fruit development. The change tendency was consistent with that of malate dehydrogenase , But the magnitude of gene expression was less than that of enzyme activity.