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目的研究在亚急性行期移植PlGF基因转染的人间充质干细胞(hMSCs)对于大鼠梗塞心肌的血管新生作用。方法使用腺病毒将PlGF基因转染人间充质干细胞(hMSCs),测定体外hMSCs及PlGF-hMSCs的PlGF蛋白表达水平。大鼠心肌梗塞一周后随机分成三组,对照组(DMEM)、hSMCs移植组、PlGF-hMSCs移植组。在移植后一个月观察心肌梗塞交界区的PlGF蛋白表达水平、血管新生以及心肌梗塞体积,并进行评价。结果体外实验中,PlGF-hMSCs的PlGF蛋白表达水平显著高于hMSCs及对照组。移植后一个月,hMSCs及PlGF-hMSCs两组在缺血心肌的梗塞交界处PlGF的蛋白水平、血管新生数量都显著高于对照组;心肌梗塞体积显著低于对照组;而上述观察指标在PlGF-hMSCs移植组显著好于hMSCs移植组。结论 PlGF因子对于心肌梗塞缺血有促进血管新生,缩小心肌梗塞体积的作用,提示着PlGF-hMSCs在临床治疗心肌梗塞方面可能有着更好的治疗作用。
Objective To investigate the angiogenic effects of human fetal stem cells (hMSCs) transfected with PlGF gene in the subacute stage on myocardial infarction in rats. Methods PlGF gene was transfected into human mesenchymal stem cells (hMSCs) by adenovirus and the expression of PlGF protein in hMSCs and PlGF-hMSCs was measured. One week after myocardial infarction, rats were randomly divided into three groups: control group (DMEM), hSMCs transplantation group and PlGF-hMSCs transplantation group. One month after transplantation, the expression of PlGF protein, angiogenesis and myocardial infarction volume were observed at the junction of myocardial infarction and evaluated. Results In vitro, PlGF protein expression of PlGF-hMSCs was significantly higher than that of hMSCs and control. One month after transplantation, the levels of PlGF protein and angiogenesis in hMSCs and PlGF-hMSCs at the infarct junction of ischemic myocardium were significantly higher than those in the control group; the infarct volume was significantly lower than that in the control group; -hMSCs transplantation group was significantly better than hMSCs transplantation group. Conclusions PlGF can promote angiogenesis and reduce the volume of myocardial infarction in myocardial infarction. It suggests that PlGF-hMSCs may have a better therapeutic effect in clinical treatment of myocardial infarction.