目的　探讨脂质体ｒＩＬ２ 对鼠脾淋巴细胞增殖及杀伤活性的作用。方法：参照Ｋｏｎｎｏ 及我所建立的方法制备复层大囊泡ｒＩＬ２ 脂质体（ ＭＬＶｒＩＬ２） 。选昆明鼠随机分成ＭＬＶｒＩＬ２ 组、ｒＩＬ２ 组、空白ＭＬＶ 组和对照组，分别腹腔注射１－５ 万Ｕ·ｋｇ１的ＭＬＶｒＩＬ２ 、１－５ 万Ｕ·ｋｇ１ 的ｒＩＬ２ 和等体积空白ＭＬＶ，连续７ 日，停药３ 日处死鼠无菌摘取脾。制取脾淋巴细胞悬液，掺入３ＨＴｄＲ 培养后测其３ＨＴｄＲ 活性，反映脾淋巴细胞增殖情况。同时选新鲜制取的脾淋巴细胞为效应细胞，Ｐ８１５细胞为靶细胞，提前使Ｐ８１５细胞掺入３ＨＴｄＲ，在效∶靶＝ ２５∶１ 和效∶靶＝ ５０∶１ 情况下使效、靶细胞共同孵育，收集残留的Ｐ８１５ 细胞并测其放射活性，反映脾淋巴细胞的杀伤活性。结果：ＭＬＶｒＩＬ２ 组、ｒＩＬ２ 组和空白ＭＬＶ 组３ＨＴｄＲ 掺入量（ ×１０３） 分别为１４－４３ ±１－２１ 、１０－７４ ±１－４９ 和９－８３ ±１－０８ 、ＭＬＶｒＩＬ２ 组最多，与各组比较 Ｐ＜ ０ ．００１ ；Ｐ８１５ 细胞中３ＨＴｄＲ 残留活性（ ×１０３） 在效∶靶＝ ５０∶１ 时，以上三组分别为１３－９５ Objective To investigate the effect of liposome rIL2 on proliferation and cytotoxicity of murine spleen lymphocytes. Methods: According to Konno and I established the method of preparation of large vesicle rIL 2 liposomes (MLV  rIL  2). Kunming mice were randomly divided into MLV  rIL  2 group, rIL  2 group, blank MLV group and control group, respectively, intraperitoneal injection of 1-5 million U · kg  1 MLV  rIL  2, 1-5 million U · kg  1 rIL  2 and equal volume of blank MLV, on the 7th, the withdrawal of rats on March 3 aseptic removal of the spleen. Preparation of spleen lymphocyte suspension, 3H-TdR incorporation of 3H-TdR activity measured after incubation, reflecting the proliferation of splenic lymphocytes. At the same time choose freshly prepared spleen lymphocytes as effector cells, P815 cells as target cells, P815 cells in advance incorporated 3H  TdR, in effect: target = 25: 1 and effect: target = 50: 1 effect, The target cells were incubated together to collect residual P815 cells and measure their radioactivity, reflecting the cytotoxic activity of spleen lymphocytes. Results: 3HTdR incorporation (× 103) in MLVrIL2 group, rIL2 group and blank MLV group were 14-43 ± 1-21, 10-74 ± 1-49 and 9-83 ± 1 -08, MLV  rIL  2 group most, compared with each group P <0. 001; 3H-TdR residual activity in P815 cells (× 103) at the effect: target = 50: 1, the above three groups were 13-95