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探讨雌激素在动脉粥样硬化形成过程中的角色,特别是研究雌二醇(estradiol)对氧化低密度脂蛋白(ox-LDL)诱导的鼠单核/巨噬细胞源性泡沫细胞J774a.1中胆固醇代谢的影响。以鼠单核/巨噬细胞J774a.1作为研究对象,分为空白对照组、泡沫细胞组和雌二醇组,用不同浓度的雌二醇(1、0.1和0.01μmol·L-1)对泡沫细胞进行干预。采用油红O染色观察泡沫细胞形成,胆固醇氧化酶荧光法检测泡沫细胞内胆固醇含量的变化;蛋白质印迹法、实时荧光定量PCR法(RTFQ-PCR)检测泡沫细胞表面清道夫受体(SR-BⅠ)的表达。结果表明,与对照组相比,泡沫细胞组内总胆固醇及胆固醇酯含量升高(P<0.001)、SR-BⅠ基因表达降低(P<0.01);与泡沫细胞组相比,不同浓度雌二醇干预组细胞内总胆固醇及胆固醇酯含量下降(P<0.05)、SR-BⅠ蛋白及基因表达升高(P<0.01),呈现出一定的浓度依赖性。结果提示,雌二醇通过降低泡沫细胞内胆固醇含量、上调SR-BⅠ的表达,从而抑制鼠单核/巨噬细胞源性泡沫细胞的形成。
To investigate the role of estrogen in the development of atherosclerosis. In particular, the effects of estradiol on oxidative low density lipoprotein (ox-LDL) -induced murine monocyte / macrophage-derived foam cell J774a.1 The impact of cholesterol metabolism. The murine monocytes / macrophages J774a.1 were divided into blank control group, foam cell group and estradiol group, with different concentrations of estradiol (1, 0.1 and 0.01 μmol·L-1) Foam cells are intervened. The formation of foam cells was observed by oil red O staining and the cholesterol content was detected by the cholesterol oxidase fluorescence method. The scavenger receptor (SR-BⅠ) on the foam cells was detected by Western blotting and real-time fluorescence quantitative PCR (RT-PCR) )expression. The results showed that compared with the control group, the content of total cholesterol and cholesteryl ester in foam cell group increased (P <0.001) and the expression of SR-BⅠ gene decreased (P <0.01). Compared with foam cell group, The content of total cholesterol and cholesterol ester decreased (P <0.05) and the protein and gene expression of SR-B Ⅰ increased (P <0.01) in alcohol-treated group, showing a concentration-dependent manner. The results suggest that estradiol inhibits the formation of murine monocyte / macrophage-derived foam cells by decreasing the cholesterol content in foam cells and up-regulating the expression of SR-BI.