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将克隆的CVB3VP1基因亚克隆至真核表达载体 pCEP4构建CVB3VP1的真核表达质粒 pCEP4 CVB3VP1。pCEP4 CVB3VP1转染HeLa细胞 ,蛋白印迹试验证明该表达体系可以在体外表达能为CVB3中和抗体识别的VP1蛋白。本研究为CVB基因疫苗的研究提供了实验依据
The cloned CVB3VP1 gene was subcloned into the eukaryotic expression vector pCEP4 to construct CVB3VP1 eukaryotic expression plasmid pCEP4 CVB3VP1. HeLa cells were transfected with pCEP4 CVB3VP1. Western blotting showed that this expression system could express VP1 protein recognized by CVB3 neutralizing antibody in vitro. This study provides experimental evidence for the study of CVB gene vaccine