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目的:通过制备As4S4/Mn0.5Zn0.5Fe2O4复合纳米粒研究体外热化疗对宫颈癌Hela细胞株的作用。方法:采用改良的化学沉淀-浸渍法制备As4S4/Mn0.5Zn0.5Fe2O4复合纳米粒,通过扫描电镜、能谱仪、原子荧光光谱仪来表征;检测As4S4/Mn0.5Zn0.5Fe2O4复合纳米粒的释药水平及在交变磁场中的升温能力;通过MTT比色法和流式细胞仪检测化疗组、热疗组及热化疗组对宫颈癌的治疗效果。结果:制备的复合纳米粒粒径为20~40 nm;复合纳米粒体外升温能达到肿瘤的有效治疗温度(41~46℃);释药缓慢,48 h释药为13.28%。在MTT实验中,As4S4/Mn0.5Zn0.5Fe2O4复合纳米粒组细胞抑制率明显高于单纯纳米雄黄溶液组和Mn0.5Zn0.5Fe2O4联合交变磁场加热组(P<0.05);在凋亡率检测中,As4S4/Mn0.5Zn0.5Fe2O4复合纳米粒联合磁流体热疗组细胞凋亡率明显高于单纯纳米雄黄溶液组和Mn0.5Zn0.5Fe2O4联合交变磁场加热组(P<0.05)。结论:采用改良的化学沉淀-浸渍法可以成功制备As4S4/Mn0.5Zn0.5Fe2O4复合纳米粒,体外实验证明该复合纳米粒联合交变磁场热疗对宫颈癌细胞具有很强的生长抑制和诱导凋亡作用。
OBJECTIVE: To study the effect of thermochemotherapy on cervical cancer Hela cells by preparing As4S4 / Mn0.5Zn0.5Fe2O4 composite nanoparticles. Methods: As4S4 / Mn0.5Zn0.5Fe2O4 composite nanoparticles were prepared by modified chemical precipitation-impregnation method and characterized by scanning electron microscopy, energy dispersive spectroscopy and atomic fluorescence spectrometry. The release of As4S4 / Mn0.5Zn0.5Fe2O4 composite nanoparticles Level and heating capacity in alternating magnetic field. MTT colorimetry and flow cytometry were used to detect the therapeutic effect of chemotherapy group, hyperthermia group and thermochemotherapy group on cervical cancer. Results: The particle size of the prepared composite nanoparticles was 20-40 nm. The composite nanoparticles could reach the effective therapeutic temperature (41-46 ° C) in vitro by increasing the temperature in vitro. The drug release was slow and the release rate was 13.28% at 48 h. In MTT experiment, the inhibition rate of As4S4 / Mn0.5Zn0.5Fe2O4 composite nanoparticles group was significantly higher than that of pure nano-realgar solution group and Mn0.5Zn0.5Fe2O4 combined with alternating magnetic field heating group (P <0.05) , The apoptosis rate of As4S4 / Mn0.5Zn0.5Fe2O4 composite nanoparticles combined with magnetic fluid hyperthermia group was significantly higher than those of pure nano-realgar solution group and Mn0.5Zn0.5Fe2O4 combined with alternating magnetic field heating group (P <0.05). Conclusion: As4S4 / Mn0.5Zn0.5Fe2O4 composite nanoparticles can be successfully prepared by modified chemical precipitation-impregnation method. In vitro experiments show that the composite nanoparticles with alternating magnetic hyperthermia have strong growth inhibition and induction of cervical cancer cells Dead effect.