Many sulfonylurea herbicides have been used under a wide variety of agronomic conditions in numerous crops. An understanding of dissipation rate of herbicide is fundamental for predicting the fate of herbicide in soil. In order to study the sulfosulfuron persistence under different cultivation systems of wheat, a four replicated experiment was carried out in the Hashemabad Reaserch Center of Gorgan, Iran in 2010 in a split plot design with two factors. Cultivation system as the main factor consisted of six levels, including conservation tillage by Combinate, no-tillage by Baldan grain drill, conservation tillage by Chizelpacker, conservation tillage by Delta Model, surface tillage by heavy disk, and conventional tillage by moldboard plow and twice disk. Secondary factor included two levels of sulfosulfuron application(with and without sulfosulfuron). Soil samples were taken at 6 stages and soil microbial respiration and soil pH were measured as factors affecting sulfosulfuron persistence. Results showed the least time of sulfosulfuron persistence belonged to the cultivation system of no-tillage by Baldan grain drill with a half-life of 4.62 d. Then, conservation tillage by Combinate and conventional tillage with a half-life of 6.30 d and conservation tillage by Delta Model with a half-life of 9.90 d were ordered. The most time of sulfosulfuron persistence(11.55 d) was related to conservation tillage by Chizelpacker. Ninety percent reduction of sulfosulfuron concentration occurred 15.34, 20.92, 32.88, and 36.38 d after sulfosulfuron application, respectively, for no-tillage system, conservation tillage by Combinate and conventional tillage, conservation tillage by Delta Model and surface tillage, and conservation tillage by Chizelpacker. In all the cultivation systems, toxicity symptoms were not observed 40 d after spraying sulfosulfuron onto the tomato plants which were used as test plant. Effects of different cultivation systems on soil microbial respiration were also significant. Many sulfonylurea herbicides have been used under a wide variety of agronomic conditions in numerous crops. An understanding of dissipation rate of herbicide is fundamental for predicting the fate of herbicide in soil. In order to study the sulfosulfuron persistence under different cultivation systems of wheat, a four replicated experiment was carried out in the Hashemabad Reaserch Center of Gorgan, Iran in 2010 in a split plot design with two factors. Cultivation system as the main factor consisted of six levels, including conservation tillage by Combinate, no-tillage by Baldan grain drill , conservation tillage by Chizelpacker, conservation tillage by Delta Model, surface tillage by heavy disk, and conventional tillage by moldboard plow and twice disk. Secondary factor included two levels of sulfosulfuron application (with and without sulfosulfuron). Soil samples were taken at 6 stages and soil microbial respiration and soil pH were measured as factors affecting sulfosulfuron persisten ce. Results showed the least time of sulfosulfuron persistence belonged to the cultivation system of no-tillage by Baldan grain drill with a half-life of 4.62 d. Then, conservation tillage by Combinate and conventional tillage with a half-life of 6.30 d and conservation tillage by Delta Model with a half-life of 9.90 d were ordered. The most time of sulfosulfuron persistence (11.55 d) was related to conservation tillage by Chizelpacker. Ninety percent reduction of sulfosulfuron concentration occurred 15.34, 20.92, 32.88, and 36.38 d after sulfosulfuron application, respectively, for no-tillage system, conservation tillage by Combinate and conventional tillage, conservation tillage by Delta Model and surface tillage, and conservation tillage by Chizelpacker. In all the cultivation systems, toxicity symptoms were not observed 40 d after spraying sulfosulfuron onto the tomato plants which were used as test plant. Effects of different cultivation systems on soil microbial respiration were also significant.