论文部分内容阅读
目的：探讨ｐ５３ 蛋白、增殖细胞核抗原（ Ｐ Ｃ Ｎ Ａ）在原发性胆囊癌组织中的表达及临床意义。方法：应用免疫组化 Ｌ Ｓ Ａ Ｂ法，对４６ 例胆囊癌及３０ 例慢性胆囊炎组织中ｐ５３ 蛋白、 Ｐ Ｃ Ｎ Ａ 的表达进行检测。结果：４６ 例胆囊癌组织中ｐ５３ 蛋白、 Ｐ Ｃ Ｎ Ａ 的阳性检出率分别为 ４７．８３％ （２２／４６）和 ６３．０４％ （２９／４６）；３０ 例慢性胆囊炎组织中分别为 ０％ （０／３０）和２３．３３％ （７／３０），癌与炎性病变之间有明显差异（ Ｐ＜ ０．０１）。高分化腺癌ｐ５３ 蛋白、 Ｐ Ｃ Ｎ Ａ 的阳性检出率分别为 ６８．１８％ （１５／２２）和８６．３６％ （１９／２２），明显高于低分化腺癌，二者有显著性差异（ Ｐ＜ ０．０１）。结论：（１）抑癌基因ｐ５３ 突变与 Ｐ Ｃ Ｎ Ａ 过度表达在胆囊癌的发生、发展过程中具有重要作用；（２）ｐ５３ 蛋白、 Ｐ Ｃ Ｎ Ａ 的过度表达与癌组织分化程度有关，可作为临床上判断胆囊癌预后的可靠生物指标。
Objective: To investigate the expression and clinical significance of p53 protein and proliferating cell nuclear antigen (PCNA) in primary gallbladder carcinoma. Methods: The expression of p53 protein and PCNA in 46 cases of gallbladder carcinoma and 30 cases of chronic cholecystitis were detected by immunohistochemical L S A B method. RESULTS: The positive detection rates of p53 protein and PCNA in 46 cases of gallbladder carcinoma were 47.83% (22/46) and 63.04% (29/46) respectively; 30 cases of chronic cholecystitis were At 0% (0/30) and 23.33% (7/30), there was a significant difference between cancerous and inflammatory lesions (P < 0.01). The positive detection rates of p53 protein and PCNA in well-differentiated adenocarcinoma were 68.18% (15/22) and 86.36% (19/22), respectively, which were significantly higher than those in poorly differentiated adenocarcinoma. Sexual differences (P < 0.01). Conclusions: (1) Tumor suppressor gene p53 mutation and P C N A overexpression have an important role in the occurrence and development of gallbladder carcinoma; (2) The overexpression of p53 protein and P C N A is related to the degree of cancer differentiation. It can be used as a reliable biological indicator to judge the prognosis of gallbladder cancer.