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用荧光染色(FDA/EB)法,染直接切刮涂片,检测了58例正在治疗的细菌仍为阳性的多菌型麻风病人。染剂的最终浓度为FDA10μg/ml,EB8μg/ml。绿染者为活菌,桔红色的为死菌,清晰可辨。治疗期8～12个月者绿菌占8.7％,13～24个月者9.4％,25～48个月者6.0％,>48个月者4.0％。后三组共55例,有8例未见绿菌,其中只用三联疗法的5例,平均治疗期16.6个月,绿菌占14.6％。提示治疗后活菌减少,有少数病人活菌可能已消失,只剩下死菌。将涂片放在室温中24小时后活菌即近乎消失,在60℃烤箱中放置30～40分钟,活菌即减少到1％±;保存在-20℃冰箱中17天,绿菌未见减少。因此可以认为用FDA/EB染色鉴别麻风菌的死活很可靠,快速、简便,可以客观地记录,值得推广,用作麻风疗效观察。
Fluorescent staining (FDA / EB) was used to stain direct incisional smears and 58 patients with multi-bacteria leprosy that were still positive were examined for the disease. The final concentration of dye is FDA10μg / ml, EB8μg / ml. Green dye those who live, orange dead, clearly identifiable. Treatment of 8 to 12 months accounted for 8.7% of green bacteria, 13 to 24 months, 9.4%, 25 to 48 months, 6.0%,> 48 months, 4.0%. The latter three groups of 55 cases, 8 cases did not see the green bacteria, of which only triple therapy in 5 cases, the average treatment period of 16.6 months, green bacteria accounted for 14.6%. Tips to reduce viable bacteria after treatment, a small number of live bacteria may have disappeared, leaving only dead bacteria. After 24 hours at room temperature, the viable cells almost disappeared, and the viable cells were reduced to 1% ± in the oven at 60 ° C for 30-40 minutes. After stored in -20 ° C freezer for 17 days, cut back. Therefore, FDA / EB staining can be used to identify the life and death of Leprosy, which is reliable, rapid and easy to record objectively and is worth popularizing for the observation of leprosy efficacy.