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目的探讨系统性红斑狼疮(SLE)患者血和尿中DNaseⅠ活性与肌动蛋白浓度的相关性。方法应用DNA-甲基绿比色法和ELISA间接竞争法检测65例SLE患者和30例正常人对照血、尿中的DNaseⅠ活性和肌动蛋白浓度,分析其相关关系。结果SLE组患者血清、尿DNaseⅠ活性均低于对照组(P<0.01),病情活动期组两者亦均低于缓解期组(P<0.01);肾损害组血清DNaseⅠ活性与非肾损害组比较,差异无显著性,而尿DNaseⅠ活性却降低(P<0.01);SLE组血浆肌动蛋白浓度高于对照组(P<0.05),但尿肌动蛋白浓度两组间差异无显著性;活动期患者血浆肌动蛋白浓度高于缓解期(P<0.01),但尿肌动蛋白浓度两组间差异无显著性;肾损害组血浆肌动蛋白浓度与非肾损害组比较,差异无显著性;但前者尿肌动蛋白浓度却高于后者(P<0.05)。SLE患者血浆肌动蛋白浓度与DNaseⅠ活性之间呈负相关(r=-0.35,P<0.05),而尿肌动蛋白浓度与DNaseⅠ活性之间无相关性(r =-0.23,P>0.05)。结论SLE患者血中DNaseⅠ活性与肌动蛋白浓度之间呈负相关,提示血中肌动蛋白浓度的增高可能是DNaseⅠ活性降低的原因之一。
Objective To investigate the relationship between DNase Ⅰ activity and actin concentration in blood and urine of patients with systemic lupus erythematosus (SLE). Methods DNase Ⅰ activity and actin concentration in blood and urine of 65 patients with SLE and 30 normal controls were detected by DNA-methyl green colorimetric assay and indirect ELISA by ELISA. The correlation was analyzed. Results Serum and urinary DNase Ⅰ activities in patients with SLE were significantly lower than those in control group (P <0.01), and those in active stage were lower than those in remission stage (P <0.01). Serum DNase Ⅰ activity in renal impairment group was significantly higher than that in non-renal impairment group (P <0.01). The plasma actin concentration in SLE group was higher than that in control group (P <0.05), but there was no significant difference in urine actin concentration between the two groups. The activity of plasma actin in active stage patients was higher than that in remission stage (P <0.01), but there was no significant difference in urine actin concentration between the two groups. There was no significant difference in plasma actin concentration between the two groups However, the former urine actin concentration was higher than the latter (P <0.05). There was a negative correlation between plasma actin concentration and DNase I activity in patients with SLE (r = -0.35, P <0.05), but no correlation between urine actin concentration and DNase I activity (r = -0.23, P> 0.05) . Conclusions There is a negative correlation between DNase Ⅰ activity and actin concentration in SLE patients, which suggests that elevated actin in blood may be one of the reasons for the decrease of DNase Ⅰ activity.