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目的:建立HPLC测定眼保Ⅰ号胶囊中芍药苷的含量测定方法。方法:Diamonsil C18(2)(4.6mm×250mm,5μm);流动相:乙腈-0.1%磷酸溶液(14:86);流速:1mL.min-1;检测波长:230nm;柱温:25℃。结果:芍药苷在0.044～0.261μg线性关系良好(r=0.9999),平均回收率97.8%,RSD=1.71%(n=6)。结论:本方法简单,准确,重现性好,可用于眼保Ⅰ号胶囊中芍药苷的含量检测。
OBJECTIVE: To establish a method for the determination of paeoniflorin in Yan Bao Ⅰ Capsule by HPLC. Method: Diamonsil C18 (2) (4.6mm × 250mm, 5μm); mobile phase: acetonitrile-0.1% phosphoric acid solution (14:86); flow rate: 1mL.min-1; detection wavelength: 230nm; Results: Paeoniflorin showed good linearity (r = 0.9999) at 0.044 ~ 0.261μg with an average recovery of 97.8% and RSD = 1.71% (n = 6). Conclusion: The method is simple, accurate and reproducible. It can be used for the determination of paeoniflorin in Eye Protection Capsule Ⅰ.