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霍乱毒素（CT）是一种有效的粘膜抗原,与不同抗原同时口服时,具有佐剂作用。作者用CT作佐剂,评估口服破伤风类毒素（TT）的B细胞抗原特异性应答,并检验CT和TT的免疫保护性。 取8～12周龄C57BL/6小鼠,分为4组,分别于0、7和14天给予口服免疫:第1组口服10μg TT和10μg CT;第2组口服100μg TT和10μg CT;第3组口服250μg TT和10μgCT;第4组仅口服250μgTT。 用ELISA检测小鼠粪便浸出物和血清中IgG、IgA、IgM抗TT,CT抗体水平。第2和第3组小鼠粪便浸出物中IgA抗TT抗体滴度分别为1:32和1:128,其余两组不产生抗TT抗体应答。口服CT后,小鼠粪便浸出物中均有IgA抗CT抗体应答。TT剂量不影响CT应答。第4组小鼠不产生抗CT抗体。仅在21天时诱发出弱血清IgG抗TT抗体应答。第2组小鼠,血清IgG抗TT抗体水平较高,第3组小鼠血清IgA抗TT抗体水平较高。血清抗CT应答IgG类最高,IgA类次之。第3组小鼠首剂免疫后,血清中无抗TT抗体,但2剂免疫后,可检出IgM和IgG抗
Cholera toxin (CT) is a potent mucosal antigen that has adjuvant effects when taken orally with different antigens. The authors used CT as an adjuvant to assess the B cell antigen specific response to oral tetanus toxoid (TT) and to examine the immunoprotective properties of CT and TT. C57BL / 6 mice (8-12 weeks old) were divided into 4 groups and were orally immunized at 0, 7 and 14 days respectively: group 1 received 10 μg of TT and 10 μg of CT; group 2 received 100 μg of TT and 10 μg of CT orally; Group 3 received 250 μg of TT and 10 μg CT orally; Group 4 received 250 μg of TT alone. The levels of IgG, IgA and IgM anti-TT and CT antibodies in excrement and serum of mice were detected by ELISA. The titer of IgA anti-TT antibody in the feces extracts of groups 2 and 3 was 1:32 and 1: 128, respectively, and the other two groups did not produce anti-TT antibody response. After oral CT, IgA anti-CT antibody response was present in the excrement of mice. TT dose does not affect CT response. Group 4 mice did not produce anti-CT antibodies. A weak serum IgG anti-TT antibody response elicited only at 21 days. Group 2 mice had higher level of serum anti-TT antibody IgG and higher level of serum anti-TT IgA antibody in group 3. Serum anti-CT response to the highest IgG, IgA followed. After the first group of mice in group 3 were immunized, there was no anti-TT antibody in the serum, but IgM and IgG were detected after two doses of immunization