论文部分内容阅读
植物名称:沙棘(Hippophae rhamnoides) 材料类别:(1)成年植株休眠茎段。(2)休眠枝条经水培萌发的腋芽。(3)幼嫩叶片。培养条件:所用外植体按常规方法消毒。(1)诱导茎段腋芽生长和生根的培养基为1/2B_5(无机盐减半),附加IBA0.2mg/L(单位下同)+IAA0.2或IBA0.4,蔗糖1.5％,琼脂0.46％。(2)。诱导愈伤组织和芽分化的培养基为1/2B_5(大量元素减半)附加6-BA0.1～0.5+IBA1.0～2.0。蔗糖
Plant Name: Hippophae rhamnoides Material Category: (1) adult plant dormant stem segment. (2) dormant branches bud hydroponic germination axillary buds. (3) young leaves. Culture conditions: The explants used were disinfected in the usual manner. (1) Induction of stem axillary bud growth and rooting medium 1 / 2B_5 (inorganic salt by half), add IBA0.2mg / L (unit same below) + IAA0.2 or IBA0.4, 1.5% sucrose, agar 0.46 %. (2). The medium for inducing callus and bud differentiation was 1 / 2B_5 (halves of macronutrients) with additional 6-BA0.1-0.5 + IBA1.0-2.0. sucrose