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目的 :研究肿瘤细胞分化时细胞膜上糖链结构的改变。方法 :通过蛋白酶消化提取细胞表面3 H标记N 糖链 ,结合外切糖苷酶处理 ,经序列凝集素亲和层析分析HL 6 0细胞诱导分化后细胞表面N 糖链结构。结果 :全反式维甲酸 (ATRA)和佛波醇 12 豆蔻酰 13 乙酸酯 (PMA)均引起HL 6 0细胞二天线复杂型和高甘露糖型糖链升高 ,多天线复杂型糖链减少。但对平分型 β N 乙酰氨基葡萄糖 (GlcNAc)糖基的影响却相反 ,ATRA使之减低 ,而PMA则促进合成。结论 :在分化过程中 ,HL 6 0细胞表面N 糖链从高分支向低分支的转变 ,可能是某些肿瘤分化的一个特征。平分型GlcNAc可能与细胞的粘附有关
Objective: To study the changes of sugar chain structure in cell membrane when tumor cells differentiate. Methods: 3 H-labeled N-linked carbohydrate chains were extracted by protease digestion and treated with exoglycosidase. The N-glycan structure on HL60 cells was analyzed by sequence-based lectin affinity chromatography. Results: All-trans retinoic acid (ATRA) and phorbol myristate 13 acetate (PMA) caused two antenna complex type and high mannose type oligosaccharide chain increased in HL 60 cells, cut back. However, the effect on the glycosylation of bisecting beta-N-acetylglucosamine (GlcNAc) is reversed. ATRA decreases it, while PMA promotes its synthesis. CONCLUSION: During the differentiation process, the transformation of N-glycan on HL-60 cells from high to low branching may be a feature of some tumor differentiation. Bisection GlcNAc may be related to cell adhesion