肿瘤坏死因子(TNF)含量甚微,获取困难,限制了理论研究及临床应用开发。1984年,兔鼠及人的TNF cDNA相继克隆成功,开创了TNF研究的新纪元。用基因工程生产rhTNF,条件适宜时产量可达40mg/升,无论从纯度和数量上均可满足理论研究及临床应用的需要,使得对TNF的认识有了突飞猛进的发展。 Tumor necrosis factor (TNF) content is very small, access difficulties, limiting the theoretical research and clinical application development. In 1984, TNF cDNA of rabbit and human was successfully cloned, which opened a new era of TNF research. The production of rhTNF by genetic engineering, when the condition is suitable, the output can reach 40mg / L, which can meet the need of theoretical research and clinical application both in purity and quantity, making the understanding of TNF have progressed by leaps and bounds.