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目的探索腺病毒介导的人基质细胞衍生因子-1对心肌梗死心室重构的影响。方法利用细菌内同源重组快速构建重组腺病毒质粒和制备hSDF-1α重组腺病毒(AdV-EGFP-hSDF-1α,Ad-SDF-1)。采用成年SD大鼠,经左冠状动脉前降支结扎建立急性心肌梗死模型。术后1h,将1×1010PFU AdV-SDF-1分6个点注射到心肌梗死部位及其周边区域。移植后4周测定心脏功能,随后取材、连续冰冻切片,观察心肌组织形态学、心肌梗死部位胶原含量的变化以及心室壁厚度的变化。结果AdV-SDF-1组心室壁明显增厚,胶原含量明显减少,心室膨胀程度明显降低,心脏功能改善。结论SDF-1过表达通过抑制心肌梗死部位胶原的合成与沉积,延缓心室重构,达到改善心脏功能的目的。
Objective To explore the effect of adenovirus-mediated human stromal cell derived factor-1 on ventricular remodeling in myocardial infarction. Methods Recombinant adenovirus plasmid was constructed by homologous recombination in bacteria and recombinant adenovirus (Ad-EGFP-hSDF-1α, Ad-SDF-1) was prepared. Adult SD rats were used to establish acute myocardial infarction model by ligation of the left anterior descending coronary artery. One hour after operation, 1 × 1010 PFU AdV-SDF-1 was injected into the myocardial infarction site and its peripheral area at 6 points. Cardiac function was measured 4 weeks after transplantation, followed by continuous frozen sectioning to observe myocardial tissue morphology, myocardial collagen content changes and changes in ventricular wall thickness. Results AdV-SDF-1 group was significantly thicker ventricular wall, significantly reduced collagen content, ventricular expansion was significantly reduced, cardiac function improved. Conclusion Overexpression of SDF-1 can delay the ventricular remodeling by inhibiting the synthesis and deposition of collagen in the myocardial infarction area and improve the cardiac function.