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目的：为探讨血清层粘连蛋白（Ｌａｍｉｎｉｎ，ＬＮ）与糖尿病微血管病变的关系，研究糖尿病患者血清ＬＮ的影响因素。方法：用放免法测定７６例２型糖尿病患者和３８例正常对照者的血清ＬＮ浓度。结果：糖尿病患者血清ＬＮ显著高于正常对照（分别为１６８．７６±２７．８４ｎｇ／ｍｌ和１４９．０９±２８．８４ｎｇ／ｍｌ，Ｐ＜０．０１有微血管病变患者显著高于无微血管病变患者（分别为 １７４．９７±２７．１４ｎｇ／ｍｌ和１５８．１２±２６．１２ｎｇ／ｍｌ Ｐ＜０．０２），糖尿病视网膜病变（ＤＲ，ｎ＝４１，１７５．６２±２６．５６ｎｇ／ｍｌ）和糖尿病肾病（ＤＮ， ｎ＝２８， １７９．５９±２９．６６ｎｇ／ｍｌ）患者血清 ＬＮ分别显著高于无 ＤＲ（ｎ＝３５，１６０．７１±２７．１２ｎｇ／ｍｌ）和无ＤＮ（ｎ＝４８，１６０．５３±２４．１１ｎｇ／ｍｌ）的患者（Ｐ＜０．０５，Ｐ＜０．０１）。同时伴有ＤＲ和ＤＮ患者血清ＬＮ高于单有ＤＲ或单有ＤＮ患者。多元逐步回归分析提示糖尿病患者血清ＬＮ浓度增高的程度与其糖尿病病程长短关系密切。糖尿病患者血清ＬＮ与血清ＩＶ型胶原、血清结合珠蛋白水平呈正相关（Ｒ＝０ ５３，Ｐ＜０．００１和Ｒ＝０．４０，Ｐ＜０．０１）?
Objective: To investigate the relationship between serum laminin (LN) and diabetic microangiopathy and to study the influencing factors of serum LN in diabetic patients. Methods: Serum LN concentration was measured by radioimmunoassay in 76 patients with type 2 diabetes mellitus and 38 normal controls. Results: Serum LN in diabetic patients was significantly higher than that in normal controls (168.76 ± 27.84ng / ml and 149.09 ± 28.84ng / ml respectively), P <0.01 was significantly higher in patients with microvascular disease than in those without microvascular disease (174.97 ± 27.14 ng / ml and 158.12 ± 26.12 ng / ml P <0.02, respectively), diabetic retinopathy (DR, n = 41,175.62 ± 26.56 ng / ml) and Serum LN in patients with diabetic nephropathy (DN, n = 28, 179.59 ± 29.66 ng / ml) was significantly higher than those without DR (n = 35,160.71 ± 27.12 ng / ml) , 160.53 ± 24.11ng / ml) (P <0.05, P <0.01) .Serum LN was higher in DR and DN patients than in patients with DR alone or DN alone.Multiple stepwise regression Analysis prompted diabetes serum N concentration was closely related to the duration of diabetes mellitus.Serum LN was positively correlated with serum type IV collagen and serum haptoglobin in patients with diabetes (R = 0 53, P <0.001 and R = 0.40, P <0 .01)?