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目的 检测垂体腺瘤组织及原代培养的垂体腺瘤细胞中 IL- 8的表达水平 ,探讨 IL- 8与垂体腺瘤的发生和发展的相关性 .方法 免疫组织化学法检测 32例垂体腺瘤标本中IL- 8的表达水平 .酶消化法对手术切取的垂体腺瘤组织作原代培养 ,收集细胞培养上清 ,双抗体夹心 EL ISA法测定 IL - 8含量 .对组织学染色进行图像分析 ,结合肿瘤的侵袭性、病理分级等进行统计学检验 .结果 所有垂体腺瘤标本均为 IL - 8阳性 ,侵袭型垂体腺瘤的 IL- 8阳性细胞明显多于非侵袭型垂体腺瘤 (P<0 .0 5 ) ,IL - 8阳性染色强度也明显高于非侵袭型垂体腺瘤 (P<0 .0 1) .所有原代培养的垂体腺瘤细胞均有IL- 8分泌 ,侵袭型垂体腺瘤细胞上清液中 IL- 8的含量明显高于非侵袭型垂体腺瘤 (P<0 .0 1) . IL - 8的表达和分泌水平与患者性别、年龄以及分级无明显相关性 .结论 IL- 8的表达和分泌可能与垂体腺瘤的侵袭性有关 ,可作为检测垂体腺瘤侵袭性高低的指标之一
Objective To detect the expression of IL-8 in pituitary adenoma tissue and primary cultured pituitary adenoma cells and to explore the correlation between IL-8 and the occurrence and development of pituitary adenoma.Methods Immunohistochemical method was used to detect the expression of IL-8 in 32 cases of pituitary adenoma The expression level of IL-8 in specimens was detected by enzyme digestion method, and the cell culture supernatants were harvested by the enzyme digestion method.The content of IL-8 was determined by sandwich enzyme-linked immunosorbent assay (ELISA) and image analysis , And statistical analysis was made according to tumor invasion, pathological grade, etc. Results All specimens of pituitary adenoma were positive for IL - 8 and IL - 8 positive cells in invasive pituitary adenoma were more than those in non - invasive pituitary adenoma (P <0. 05), and the positive staining intensity of IL - 8 was also significantly higher than that of non - invasive pituitary adenoma (P <0.01) .All the primary cultured pituitary adenoma cells had IL-8 secretion and invasive type The level of IL-8 in pituitary adenoma cell supernatant was significantly higher than that in non-invasive pituitary adenoma (P <0.01). There was no significant correlation between the level of IL-8 expression and secretion in patients with gender, age and grade Conclusion The expression and secretion of IL-8 may be related to pituitary gland Invasive tumor related, can be used as an indicator of invasiveness of pituitary adenoma