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采用经改进的蛋白质双向电泳技术 ,分析了水稻温敏叶绿素突变体武金 4 B和 110 3S“斑马叶”性状表达过程中叶片蛋白质的变化。结果表明 :在该性状表达期 ,叶片蛋白质含量下降 ,特别是Rubis CO大、小亚基含量明显减少 ;在叶片的失绿部分蛋白 P1(MW5 6k D,p I6.7)发生特异缺失 ;在叶片的绿色部分该蛋白明显表达。复绿后 ,P1蛋白正常表达。在对照及未经变温刺激的武金 4 B叶片中该蛋白表达明显。由此推测 :P1蛋白可能是一种与叶绿素代谢过程密切相关的重要功能蛋白。此外 ,本文还就这种“斑马叶”性状应用于水稻育种实践的可能性进行了讨论
The improved protein two dimensional electrophoresis technique was used to analyze the changes of leaf protein during the expression of the leaf traits of Wujin 4 B and 110 3S “zebra leaves”. The results showed that the content of protein in leaves was decreased at the expression stage of the traits, especially the contents of large and small subunits of Rubis CO were obviously decreased. The specific deletion of protein green protein (MW5 6k D, p I6.7) The green part of the leaf is clearly expressed. After re-green, P1 protein is normally expressed. The protein expression was significant in the control and non-temperature-stimulated Wujin 4 B leaves. It is speculated that P1 protein may be an important functional protein closely related to chlorophyll metabolism. In addition, the paper also discussed the possibility of application of this “zebra leaf” trait in rice breeding practice