论文部分内容阅读
目的:测定比合玛尔江散中没食子酸的含量。方法:采用高效液相色谱法,Pheomenex luna C18色谱柱(250×4.60mm,5μm),柱温35℃,流动相甲醇-0.2%磷酸(5:95)为流动相,检测波长273nm,流速1.0mL/min结果:没食子酸在21.88~218.80μg/mL间呈良好的线性关系,r=0.9997,平均回收率为102.11%,RSD0.53%。结论:该方法具有操作简单,灵敏,准确的优点,分离效果显著,可用于余甘子的含量测定和本产品质量控制。
OBJECTIVE: To determine the content of gallic acid in Bimamarjiang powder. Methods: The mobile phase consisted of Pheomenex luna C18 column (250 × 4.60mm, 5μm), column temperature 35 ℃, mobile phase methanol -0.2% phosphoric acid (5:95), detection wavelength at 273nm, flow rate of 1.0 mL / min. Results: There was a good linear relationship between gallic acid and 21.88 ~ 218.80μg / mL, r = 0.9997. The average recovery was 102.11% and RSD was 0.53%. Conclusion: The method has the advantages of simple operation, sensitive and accurate. The separation effect is significant. It can be used for the determination of Phyllanthus emblica and the quality control of this product.