DNA重组技术已广泛用于疟疾的研究,用含有疟原虫DNA重复序列的重组质粒作探针检测疟原虫,具有较好的敏感性和特异性,在疟疾的流行病学调查和疟原虫虫株分类等方面有重要意义。目前,DNA探针的标记物大多采用放射性同位素,对人体有辐射危害,难以在疟疾流行区现场推广应用。作者试用生物素标记两种非洲地理株恶性疟原虫 DNA recombinant technology has been widely used in the study of malaria, the recombinant plasmid containing the DNA sequence of Plasmodium was used as a probe to detect the malaria parasite, which has good sensitivity and specificity. In the epidemiological survey of malaria and Plasmodium strain Classification and other aspects of great significance. At present, most of the DNA probe labels use radioisotopes, which are harmful to human body and difficult to popularize in the malaria epidemic area. The authors tried biotin to label two African geographic strains of Plasmodium falciparum