Dichloromethane is harmful to human health and hazardous to atmospheric environment. In this study, two strains were isolated which were identified as Pseudomonas sp.and Mycobacterium sp., and utilized dichloromethane (DCM) as sole carbon and energy sources. The optimal culture conditions were temperature of 28℃ and pH of 6.5 for obtaining the two mixed bacterial strains.The investigation on the purification of DCM-contaminated gas was carried out in a bench-scale biotrickling filter which was inoculated with the two strains and operated under these optimal conditions. The DCM removal efficiencies varied between 72% and 99% in the biotrickling filter when empty-bed residence time was 9.6 s with the inlet concentrations ranged from 0.7 to 3.12 g/m3 under the conditions of pH of 6.5±0.5 and temperature of 28℃. It was also found that NaCl accumulation in the broth would inhibit the DCM biodegradation dramatically when the accumulated NaCl concentration was over 35.1 g/L.