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目的:探讨FPN1基因多态性与HiHiLo训练效果的关联性。方法:选取70名健康受试者在模拟海拔2 800 m高度进行30 d HiHiLo训练(低氧暴露10 h/d、除白天常氧训练外,以75%.VO2max强度每周进行3次低氧训练),测定训练前、训练后第4 d、第7 d、第16 d、第23 d和第30 d的RBC、Hct和Hb等血象指标,应用PCR-RFLP方法解析基因多态性。结果:FPN1基因rs1123110和rs4145237多态性与血象指标的初始值不关联。经30 d HiHiLo训练训练后,rs1123110位点TT型RBC、Hb、Hct的平均增加量显著高于TC型;TT型Hb、Hct从第7 d、RBC从第16 d开始显著增加并持续至第30 d。rs4145237位点GG型RBC、Hb、Hct的平均增加量显著高于CG型;GG型Hb、Hct、RBC从第16 d开始显著增加,随后持续升高至第30 d。结论:rs1123110的TT型和rs4145237的GG型与HiHiLo训练效果关联,可以作为预测血象指标训练效果的分子标记。
Objective: To investigate the association between FPN1 gene polymorphism and HiHiLo training effect. Methods: Seventy healthy volunteers were trained on HiHiLo for 30 days at an altitude of 800 m (hypoxia exposure 10 h / d, except for nocturnal aerobic training, 75% Training) to determine the hemoglobin levels of RBC, Hct and Hb before training, 4 d, 7 d, 16 d, 23 d and 30 d after training, and then analyze the gene polymorphism by PCR-RFLP. Results: The polymorphisms of rs1123110 and rs4145237 in FPN1 gene were not correlated with the initial value of blood index. After 30 days of HiHiLo training and training, the average increase of TT type RBC, Hb and Hct at rs1123110 was significantly higher than that of TC type. From the 7th day of type TT, the Rct increased significantly from the 16th day to the 30 d. The mean increase of RBC, Hb and Hct of GG at rs4145237 was significantly higher than that of CG. The HG, Hct and RBC of GG increased significantly from the 16th day, and then continued to rise to the 30th day. Conclusion: TT genotypes of rs1123110 and genotype GG of rs4145237 are correlated with the training effect of HiHiLo, which can be used as a molecular marker for predicting the training effect of blood indicators.