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目的和方法研究7_hydroxystaurosporine(UCN_01)对转移性前列腺肿瘤DU_145细胞浸润和转移的影响。用体外转移和创伤法检查细胞浸润和转移 ,用Westernblotting 法检查蛋白质的表达。结果UCN_01在非毒性剂量下(100nmol·L -1)明显地抑制DU_145细胞浸润和转移。而且 ,UCN_01这种抗肿瘤浸润和转移功能与它增加细胞_细胞粘连分子E_cadherion的表达有关。结论这些实验首次证实UCN_01能抑制人前列腺肿瘤细胞的浸润和转移。UCN_01的临床应用可能更有效地控制前列腺肿瘤转移
Objectives and Methods To investigate the effect of 7_hydroxystaurosporine (UCN_01) on the invasion and metastasis of DU_145 cells in metastatic prostate tumors. Cell infiltration and metastasis were examined by in vitro transfer and trauma, and protein expression was examined by Western blotting. Results UCN_01 significantly inhibited DU_145 cell infiltration and metastasis at a nontoxic dose (100 nmol·L -1 ). Moreover, the anti-tumor infiltration and metastasis of UCN_01 is associated with its increased expression of cell adhesion molecule E_cadherion. Conclusion These experiments demonstrated for the first time that UCN_01 can inhibit the infiltration and metastasis of human prostate tumor cells. Clinical application of UCN_01 may be more effective in controlling prostate tumor metastasis