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目的:探讨植物乳杆菌LIP-1的降血脂作用及其机理。方法:将24只雄性Wistar大鼠按体重分为正常对照组、高脂模型组及实验组,其中后两组给予高脂饲料喂养,并每日给上述3组分别灌胃0.9%生理盐水、脱脂乳和活菌制剂,28d后测定相关指标。结果:实验组血清总胆固醇、低密度脂蛋白胆固醇、甘油三酯和丙二醛含量明显低于高脂模型组(P<0.05),而血清高密度脂蛋白胆固醇含量和血清的谷胱甘肽过氧化物活力明显高于高脂模型组(P<0.05);肝脏组织匀浆中的谷胱甘肽过氧化物酶活力明显高于高脂模型组(P<0.05),丙二醛含量明显低于高脂模型组(P<0.05),肝脏中的胆固醇含量明显低于高脂模型组(P<0.05),粪便中的胆固醇及短链脂肪酸含量明显高于高脂模型组(P<0.05)。结论:植物乳杆菌LIP-1对高脂血症大鼠有明显的降血脂作用,其作用机理涉及抗氧化作用在内的多种途径。
Objective: To investigate the hypolipidemic effect of Lactobacillus plantarum LIP-1 and its mechanism. Methods: Twenty-four male Wistar rats were divided into normal control group, high fat model group and experimental group according to body weight. The latter two groups were given high-fat diet, and the above three groups were given 0.9% Skimmed milk and viable preparations, 28d after the determination of relevant indicators. Results: The contents of serum total cholesterol, low density lipoprotein cholesterol, triglyceride and malondialdehyde in the experimental group were significantly lower than those in the high fat model group (P <0.05), while the levels of serum high density lipoprotein cholesterol and serum glutathione (P <0.05). The glutathione peroxidase activity in liver homogenate was significantly higher than that in high-fat model group (P <0.05), the content of malondialdehyde was significantly higher (P <0.05), the content of cholesterol in the liver was significantly lower than that in the model group (P <0.05), and the contents of cholesterol and short chain fatty acids in the feces were significantly higher than those in the model group (P <0.05) ). Conclusion Lactobacillus plantarum LIP-1 has obvious hypolipidemic effects on hyperlipidemic rats, and its mechanism of action involves various pathways including antioxidation.