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目的：初步探讨一氧化氮对心脏功能的作用及其可能机制。方法：本实验利用Ｌａｎｇｅｎｄｏｒｆ离体工作心脏灌流装置及技术，观察了ＮＯ供体ＳＩＮ－１、硝普钠灌流心脏后心功能及心肌某些生化指标的改变情况。结果：心脏功能多项指标明显下降，以ＬＶＳＰ，±ｄｐ／ｄｔｍａｘ为甚。伴之心肌ｃＧＭＰ含量的升高及心肌细胞膜Ｎａ＋－Ｋ＋－ＡＴＰ酶活性的下降。结论：一氧化氮通过调控心肌ｃＧＭＰ的合成及肌膜Ｎａ＋－Ｋ＋－ＡＴＰ酶活性而影响心肌功能。
Objective: To investigate the effect of nitric oxide on cardiac function and its possible mechanism. Methods: In this study, Langendorf isolated heart perfusion device and technology were used to observe the change of cardiac function and myocardial biochemical indexes after NO donor SIN-1 and sodium nitroprusside perfusion. Results: Multiple indexes of cardiac function decreased significantly, especially LVSP and ± dp / dtmax. Accompanied by increased myocardial cGMP content and myocardial cell membrane Na + -K + -ATPase activity decreased. Conclusion: Nitric oxide can affect myocardial function by regulating the synthesis of myocardial cGMP and Na + -K + -ATPase activity in sarcolemma.