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目的研究亚磁场和后肢去负荷对大鼠腓肠肌介电特性的影响。方法雄性SD大鼠24只分别在正常环境(Con),亚磁场环境(HMF),后肢去负荷环境(HLU)以及亚磁场和后肢去负荷复合环境(HMF+HLU)饲养4周。用阻抗分析仪在0.1 MHz到120 MHz频率范围内检测大鼠腓肠肌介电谱,用单项Cole-Cole方程对实验数据进行拟合,获得大鼠腓肠肌的介电参数。结果 1)HMF和HLU处理后腓肠肌的电导率增加,HMF组增加程度更高。2)介电常数的虚部和电导率的虚部受HMF或HLU作用后均减小。3)受HMF或HLU影响,特征频率移向高频。4)HMF作用后腓肠肌的κ0.1MHz值显著升高(P<0.05),尽管HLU处理后Δε降低12.8%,κ0.1MHz增加30.9%,但差异不具统计性。结论 HLU处理大鼠4周对大鼠腓肠肌介电特性无显著影响,与HLU作用相比,HMF的作用更强。
Objective To study the effect of sub-magnetic field and hindlimb de-loading on the gastrocnemius dielectric properties in rats. Methods Twenty-four male Sprague-Dawley rats were housed for 4 weeks under normal environment (Con), sub-magnetic field (HMF), hindlimb-loaded environment (HLU) and sub-magnetic field and hind limb load-shedding complex environment (HMF + HLU). The impedance spectrometer was used to measure the gastrocnemius electrical spectrum of the rat in the frequency range of 0.1 MHz to 120 MHz. The experimental data were fitted with a single Cole-Cole equation to obtain the dielectric parameters of gastrocnemius in rats. Results 1) The electrical conductivity of gastrocnemius muscle increased after HMF and HLU treatment, and the HMF group increased more. 2) The imaginary part of the dielectric constant and the imaginary part of the electric conductivity decrease after the action of HMF or HLU. 3) Affected by HMF or HLU, the characteristic frequency shifts to high frequency. 4) After the HMF treatment, the gastrocnemius κ0.1MHz increased significantly (P <0.05). Although the Δε decreased 12.8% and κ0.1MHz increased 30.9% after HLU treatment, the difference was not statistically significant. Conclusion HLU treatment for 4 weeks has no significant effect on the gastrocnemius dielectric properties of rats, and the effect of HMF is stronger than that of HLU.