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目的探讨磁共振(MRI)弥散加权(DWI)成像对~(125)Ⅰ粒子组织间植入治疗人胰腺癌裸鼠移植瘤疗效的评估价值。方法将人胰腺癌SWI990细胞株接种于BABL/C裸鼠右下肢旁腹股沟区偏背侧皮下,待瘤体长至8～10 mm进行干预,共有16只裸鼠的成瘤大小适用于实验,分为实验组8只,植入~(125)Ⅰ粒子,和对照组8只,植入空载粒子。粒子植入前及治疗后2周和2个月时分别行MRI常规扫描及DWI成像。取瘤体标本行组织病理学检查。结果实验组肿瘤细胞坏死明显,而对照组肿瘤细胞无明显或有少许坏死。裸鼠心、肝、肺、肾及脾脏等组织无明显放射炎症表现。常规MRI成像评价~(125)Ⅰ粒子治疗胰腺癌疗效的价值有限。DWI显示实验组内整个肿瘤组织的表观弥散系数(ADC)值在治疗前为(0.001 15±0.000 13)mm~2/s,治疗后2周为(0.00I 29±0.000 038)mm~2/s,治疗后2个月为(0.002 08±0.000 14)mm~2/s,与治疗前相比差异有统计学意义(P<0.05)。实验组肿瘤实质区的ADC值亦较治疗前及对照组增高,但低于坏死区ADC值。结论 ~(125)Ⅰ粒子组织间植入治疗人胰腺癌裸鼠移植瘤可导致肿瘤坏死.并对周围脏器是安全的。用常规MRI及DWI成像观察裸鼠皮下移植瘤可行。DWI对疗效评估有重要价值。
Objective To investigate the value of magnetic resonance (MRI) diffusion weighted (DWI) imaging in the evaluation of the therapeutic effect of ~ (125) Ⅰ interstitial implantation in human pancreatic cancer xenografts in nude mice. Methods Human pancreatic cancer cell line SWI990 was inoculated subcutaneously in the dorsal limb of the right lower extremity in BABL / C nude mice. When the length of the tumor reached 8-10 mm, intervention was performed. The tumor size of 16 nude mice was suitable for experiment. Divided into the experimental group of 8, 125I seeds implanted, and the control group of 8, implanted empty particles. Before routine implantation and 2 weeks and 2 months after treatment, MRI and DWI were performed respectively. Tumor specimens were taken for histopathological examination. Results The experimental group of tumor necrosis was obvious, while the control group of tumor cells without obvious or a little necrosis. Nude mice heart, liver, lung, kidney and spleen and other tissues showed no significant inflammatory manifestations. The value of ~ (125) Ⅰ particles for the treatment of pancreatic cancer is limited by conventional MRI. DWI showed that the apparent ADC value of the entire tumor tissue in the experimental group was (0.001 15 ± 0.000 13) mm ~ 2 / s before treatment and (0.001 29 ± 0.000 038) mm 2 after 2 weeks of treatment / s after treatment for 2 months (0.002 08 ± 0.000 14) mm ~ 2 / s, compared with before treatment, the difference was statistically significant (P <0.05). The ADC value of the tumor parenchyma in the experimental group was also higher than that before treatment and in the control group, but lower than the ADC value in the necrotic area. Conclusion The implantation of ~ (125) Ⅰ interstitial tissue in human pancreatic cancer xenografts in nude mice can lead to tumor necrosis and is safe for the surrounding organs. It is feasible to observe the subcutaneous xenografts in nude mice by conventional MRI and DWI imaging. DWI has important value in the evaluation of the curative effect.