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本研究分两批实验观察了缺锌对大鼠维生素A代谢的影响。实验Ⅰ用24头体重55g左右的雄性Wistar大鼠,随机分成缺锌组、对喂组、加锌组,每组8头,实验期为30天,观察指标有摄食量、体重、血清锌、血清维生素A、肝脏维生素A、胫骨锌。结果表明,缺锌严重影响了大鼠生长发育,并使维生素A积聚于肝脏,血液中维生素A的浓度明显减低。实验Ⅱ用48头体重55g左右雄性Wistar大鼠,先喂缺锌饲料30天,造成缺锌状态,再按体重随机分为四组:缺锌组、治疗Ⅰ组、治疗Ⅱ组、治疗Ⅲ组。三个治疗组中每日每头大鼠腹腔注射锌量分别为0.63μmol、1.89μmol、3.85μmol,治疗期为6天,观察指标同实验Ⅰ。结果为,三个治疗组血清锌及维生素A的浓度均明显高于缺锌组,而肝脏中维生素A的浓度均明显低于缺锌组;说明补锌可以使缺锌大鼠动员肝脏中贮存的维生素A进入血液。
In this study, two batches of experiments were conducted to observe the effect of zinc deficiency on vitamin A metabolism in rats. In experiment I, 24 male Wistar rats with a body weight of about 55g were randomly divided into a zinc-deficient group and a zinc-fed group, with 8 rats in each group. The experimental period was 30 days. The observation indexes included food intake, body weight, serum zinc, Serum vitamin A, liver vitamin A, tibia zinc. The results showed that zinc deficiency seriously affected the growth and development of rats and caused the accumulation of vitamin A in the liver. The concentration of vitamin A in the blood was significantly reduced. Experiment Ⅱ Forty-eight male Wistar rats with a body weight of about 55g were fed with zinc-deficient diet first for 30 days, then were divided into four groups according to body weight: zinc deficiency group, treatment group Ⅰ, treatment group Ⅱ, treatment group Ⅲ . The intraperitoneal injection of zinc in each of the three treatment groups was 0.63μmol, 1.89μmol, 3.85μmol, respectively for 6 days. The observation indexes were the same as those in Experiment Ⅰ. The results showed that the three treatment groups serum zinc and vitamin A concentrations were significantly higher than the zinc deficiency group, while the liver vitamin A concentrations were significantly lower than the zinc deficiency group; zinc supplementation can make zinc-deficient rats mobilized liver storage Vitamin A enters the bloodstream.