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研究表明 ,血和尿中的胰蛋白酶原激活肽 (TAP)含量 ,能够特异地反映胰蛋白酶的肠外激活和胰腺坏死的程度。这对及时、准确地诊断急性胰腺炎有重要的临床意义。本研究用KLH连接的TAP作免疫原 ,共建立了 4株抗TAP的单克隆抗体杂交瘤细胞株 ,均属IgG1亚类。单克隆抗体效价达 1:5× 10 5至 1:1× 10 7。效价高 ,稳定性好 ,特异性强 ,与胰蛋白酶原无交叉反应。为建立更敏感、更特异的TAP免疫检测方法 ,并用于探讨胰蛋白酶异位激活的致病机制的研究 ,以及全身性炎症反应在局部损伤作用的研究创造了条件。
Studies have shown that trypsinogen (TAP) levels in blood and urine specifically reflect the extent of pituitary activation and pancreatic necrosis of trypsin. This is a timely and accurate diagnosis of acute pancreatitis has important clinical significance. In this study, KLH-linked TAP as an immunogen, a total of four anti-TAP monoclonal antibody hybridoma cell lines, are IgG1 subclass. Monoclonal antibody titer of 1: 5 × 10 5 to 1: 1 × 10 7. High potency, good stability, specificity, no cross-reaction with trypsinogen. In order to establish a more sensitive and specific TAP immunoassay method, and to explore the pathogenesis of trypsin ectopic activation, and the role of systemic inflammation in the study of local injury to create the conditions.