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目的:建立测定妇炎康分散片中原儿茶醛含量的HPLC法。方法:使用ODS-C18柱,在流动相为甲醇-乙腈-水-冰乙酸(5:10:85:1),检测波长为280nm,柱温为30℃的条件下,对妇炎康分散片中原儿茶醛的含量进行测定。结果:原儿茶醛在0.029-0.176mg·mL-1内质量浓度与峰面积呈良好的线性关系,线性回归方程为A=1.87×105C-90,r=0.9999,平均回收率为100.1%(RSD=0.48%)。结论:该法准确度高、专属性好,可用于妇炎康分散片的含量测定。
Objective: To establish HPLC method for determination of protocatechuic aldehyde content in fuyankang dispersible tablets. METHODS: The ODS-C18 column was used to disperse Fu Yankang under the conditions of methanol-acetonitrile-water-glacial acetic acid (5:10:85:1) as mobile phase, detection wavelength of 280 nm, and column temperature of 30°C. The content of catechins in tea was determined. Results: There was a good linear relationship between the concentration of protocatechuic aldehyde in the range of 0.029-0.176 mg·mL-1 and the peak area. The linear regression equation was A=1.87×105C-90, r=0.9999, and the average recovery was 100.1% ( RSD = 0.48%). Conclusion: The method has high accuracy and specificity and can be used for the determination of Fuyankang dispersible tablets.