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目的 观察环孢素Ａ（ＣｓＡ） 、霉酚酸（ ＭＰＡ） 和雷帕霉素（ＲＰＭ） 对ＣＤ２８ 通路共刺激后淋巴细胞ＩＬ４ ｍ ＲＮＡ 表达的影响。 方法 以ＰＣＲ 扩增方法获得ＩＬ４ 特异的ｃＤＮＡ 片段，并将其克隆至ＰＧＥＭ３Ｚ 载体中。以此作为探针对ＲＴＰＣＲ 产物进行杂交定量，比较三种免疫抑制剂对ＣＤ２８通路共刺激后淋巴细胞ＩＬ４ ｍ ＲＮＡ 表达的影响。 结果 以抗ＣＤ３ ｍ Ａｂ ＋ 抗ＣＤ２８ ｍ Ａｂ 共刺激后，发现淋巴细胞稳定地表达ＩＬ４ ｍ ＲＮＡ；ＣｓＡ 和ＭＰＡ 未能抑制淋巴细胞ＩＬ４ ｍ ＲＮＡ 的表达，而ＲＰＭ则能产生显著抑制。 结论 ＲＰＭ 能抑制ＣＤ２８ 通路共刺激后ＩＬ４ ｍ ＲＮＡ 的表达，而ＣｓＡ 和ＭＰＡ对这一过程无抑制作用。
Objective To observe the effects of cyclosporin A (CsA), mycophenolic acid (MPA) and rapamycin (RPM) on the expression of IL4 mRNA in lymphocytes co-stimulated by CD28. Methods IL-4-specific cDNA fragments were obtained by PCR amplification and cloned into PGEM3Z vector. As a probe for RT PCR products hybridization quantitative comparison of three immunosuppressive agents CD28 pathway co-stimulated lymphocyte IL 4 m RNA expression. Results After co-stimulation with anti-CD3 m Ab + anti-CD28 m Ab, lymphocytes stably expressed IL-4 m RNA; CsA and MPA failed to inhibit the expression of IL-4 m RNA in lymphocytes, while RPM produced significant inhibition . Conclusion RPM can inhibit the expression of IL-4 mRNA after co-stimulation of CD28 pathway, while CsA and MPA have no inhibitory effect on this process.