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目的 观察环孢素A(CsA) 、霉酚酸( MPA) 和雷帕霉素(RPM) 对CD28 通路共刺激后淋巴细胞IL4 m RNA 表达的影响。 方法 以PCR 扩增方法获得IL4 特异的cDNA 片段,并将其克隆至PGEM3Z 载体中。以此作为探针对RTPCR 产物进行杂交定量,比较三种免疫抑制剂对CD28通路共刺激后淋巴细胞IL4 m RNA 表达的影响。 结果 以抗CD3 m Ab + 抗CD28 m Ab 共刺激后,发现淋巴细胞稳定地表达IL4 m RNA;CsA 和MPA 未能抑制淋巴细胞IL4 m RNA 的表达,而RPM则能产生显著抑制。 结论 RPM 能抑制CD28 通路共刺激后IL4 m RNA 的表达,而CsA 和MPA对这一过程无抑制作用。
Objective To observe the effects of cyclosporin A (CsA), mycophenolic acid (MPA) and rapamycin (RPM) on the expression of IL4 mRNA in lymphocytes co-stimulated by CD28. Methods IL-4-specific cDNA fragments were obtained by PCR amplification and cloned into PGEM3Z vector. As a probe for RT PCR products hybridization quantitative comparison of three immunosuppressive agents CD28 pathway co-stimulated lymphocyte IL 4 m RNA expression. Results After co-stimulation with anti-CD3 m Ab + anti-CD28 m Ab, lymphocytes stably expressed IL-4 m RNA; CsA and MPA failed to inhibit the expression of IL-4 m RNA in lymphocytes, while RPM produced significant inhibition . Conclusion RPM can inhibit the expression of IL-4 mRNA after co-stimulation of CD28 pathway, while CsA and MPA have no inhibitory effect on this process.