本文根据动物实验观察,报导精制抗银环蛇毒血清、胰蛋白酶及糜蛋白酶对银环蛇毒中毒动物保护作用的比较,为临床合理选用或合用这些药物提供参考。一、实验材料(一) 银环蛇毒(下文简称蛇毒);本教研室自行采集。(二) 精制抗银环蛇毒血清(下文简称抗血清):本教研室与上海生物制品研究所协作研制。(三) 胰蛋白酶:上海生物化学制药厂生产供注射用结晶胰蛋白酶,每一安瓿为2,000u。(四) 糜蛋白酶:上海生物化学制药厂生产供注射用结晶糜蛋白酶,每一安瓿5mg。(五) 实验动物:体重18～22g小白鼠,雌雄兼用。二、实验方法将蛇毒结晶用蒸馏水深解配成1 mg/1ml母液,按实验需要再配成3.5 μg/0.1 ml、7μg/0.1 ml、10.5μg/0.1ml及14μg/0.1ml四种蛇毒溶液(这批蛇 Based on the observation of animal experiments, this article reports the comparison of the protective effects of purified anti-silver venom serum, trypsin and chymotrypsin on the animal protection of the snake venom poisoning, and provides a reference for clinically rational selection or combination of these drugs. First, the experimental materials (a) silver ring snake venom (hereinafter referred to as snake venom); the teaching staff to collect on their own. (b) Purification of anti-silver venom serum (hereinafter referred to as antiserum): The teaching and research section and the Shanghai Institute of Biological Products have collaborated in the development. (3) Trypsin: The production of crystalline trypsin for injection by Shanghai Biochemical Pharmaceutical Factory is 2,000u for each ampoule. (IV) Chymotrypsin: Shanghai Biochemical Pharmaceutical Factory produces crystalline chymotrypsin for injection, 5 mg per ampoule. (5) Experimental animals: mice weighing 18-22 g, both male and female. Second, the experimental method will snake crystallization crystallized with distilled water into 1 mg/1ml mother liquor, according to the experimental need to be formulated into 3.5 μg/0.1 ml, 7μg/0.1 ml, 10.5μg/0.1ml and 14μg/0.1ml four snake poison solution (The snakes