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茶多酚(TP)是从天然植物茶叶中分离提纯的多酚类化合物复合体,具有护肝、抗氧化和免疫调节等多方面的作用。拟通过观察茶多酚对酒精性肝损伤动物模型的保护作用及白细胞介素-10(IL-10)在动物肝脏中的表达,探讨IL-10在酒精性肝病发病中的作用以及茶多酚防治酒精性肝病的机制。一、材料与方法1.材料:清洁级SD大鼠86只,体重180-210 g,由浙江大学医学院动物中心提供,以清洁Ⅱ级标准饲养;体积分数56% 白酒,北京酿酒总厂生产;苏木素染料、伊红染料,由上海试剂三厂生产;羊抗大鼠IL-10多克隆抗体和ABC法免疫组织化学试剂盒,由美国Santa Cruz公司生产。
Tea polyphenols (TP) is a polyphenol compound complex isolated and purified from natural plant tea, and has various functions such as protecting the liver, antioxidation and immune regulation. To observe the protective effect of tea polyphenols on alcoholic liver injury animal model and the expression of interleukin-10 (IL-10) in animal liver, and to explore the role of IL-10 in the pathogenesis of alcoholic liver disease and the effects of tea polyphenols Prevention and treatment of alcoholic liver disease mechanism. MATERIALS AND METHODS 1. Materials: Totally 86 SD grade rats, weighing 180-210 g, were provided by Animal Center of Zhejiang University School of Medicine and were kept clean grade II. The volume fraction of 56% white spirit was produced by Beijing Brewery ; Hematoxylin dye, Eosin dye, produced by the Shanghai Reagent Factory; Goat anti-rat IL-10 polyclonal antibody and ABC method immunohistochemistry kit produced by the United States Santa Cruz.