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目的:制备无患子皂苷抑菌活性部位。方法:以无患子皂苷的洗脱率、精制度为指标,考察大孔吸附树脂对无患子皂苷的吸附性能和洗脱参数,并采用管碟法评测各洗脱部位抑菌活性强弱,以确证无患子皂苷制备工艺的合理性。结果:13.6mL无患子皂苷样品液(生药0.01g/mL)上大孔吸附树脂柱(Φ15mm×H90mm,干重2.5g),用蒸馏水、30%乙醇、70%乙醇各3BV依次洗脱,抑菌活性部位无患子皂苷富集于70%乙醇洗脱液中。结论:通过大孔吸附树脂富集与纯化,无患子皂苷洗脱率为93.8%,精制度为250.1%,为无患子抑菌活性部位的开发提供了物质保障,亦为其它皂苷类化合物的纯化工艺研究提供了实验参考。
Objective: To prepare the saponin active ingredient. Methods: The elution rate and the degree of purification of Sapindus saponins were used as indexes to investigate the adsorption properties and elution parameters of Saponins of Macroporous Adsorption Resin. The inhibitory activity of Saponins , To confirm the saponin saponin preparation process is reasonable. Results: 13.6mL Saponin sample crude drug (crude drug 0.01g / mL) on the macroporous resin column (Φ15mm × H90mm, dry weight 2.5g), with distilled water, 30% ethanol, Antibacterial active site Saponins enriched in 70% ethanol eluent. Conclusion: The enrichment and purification of macroporous adsorption resin showed that the elution rate of apis saponins was 93.8% and the purification rate was 250.1%, which provided the material guarantee for the development of the active site of antibacterial activity against Sapindus citricola and other saponins Purification process provides an experimental reference.