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目的：了解Ｂ７分子在体外抗肝癌免疫中的作用。方法：健康人外周血单个核细胞（ＰＢＭＣ）与ＨｅｐＧ２／ｈＢ７－１。ＨｅｐＧ２／ｎｅｏ及亲代ＨｅｐＧ２瘤苗混合培养（ＭＬＴＣ），检测淋巴细胞活化增殖能力，淋巴细胞ＨＬＡ－１类抗原的表达，培养上清ＩＦＮ－γ水平、ＴＮＦ活性及ＬＡＫ、ＣＴＬ细胞活性。结果：ＨｅｐＧ２／ｈＢ７－１瘤苗促淋巴细胞增殖最高达１４．６倍，明显高于ＨｅｐＧ２／ｎｅｏ和ＨｅｐＧ２瘤苗的作用（Ｐ＜０．０５）。ＨｅｐＧ２／ｈＢ７－１瘤苗刺激后淋巴细胞ＨＬＡ－Ｉ类抗原表达、ＩＦＮ－γ及ＴＮＦ分泌均高于ＨｅｐＧ２／ｎｅｏ、ＨｅｐＧ２瘤苗刺激组。Ｅ／Ｔ＝４０：１时ＬＡＫ细胞对ＨｅｐＧ２／ｈＢ７－１细胞的杀伤率为杀伤ＨｅｐＧ２／ｎｅｏ和ＨｅｏｐＧ２胞的２．０，２．４倍；ＣＴＬ细胞对ＨｅｐＧ２／ｈＢ７－１细胞杀伤为对ＨｅｐＧ２／ｎｅｏ及ＨｅｐＧ２杀伤的２．７倍，具有明显差异（Ｐ＜０．００１）。结论：抗肝癌免疫中Ｔ细胞活化也需双信号共刺激。Ｂ７－１分子能诱导体外抗肝癌免疫反应。
Objective: To understand the role of B7 in anti-HCC immunity in vitro. Methods: Peripheral blood mononuclear cells (PBMCs) and HepG2 / hB7-1 were cultured in healthy volunteers. HepG2 / neo and HepG2 inoculated with HepG2 cells were cultured in vitro. The activity of lymphocyte activation and proliferation, the expression of lymphoid HLA-1 antigen, the level of IFN-γ in culture supernatant, the activity of TNF and the activity of LAK and CTL were detected. Results: HepG2 / hB7-1 vaccine induced lymphocyte proliferation up to 14.6-fold, significantly higher than that of HepG2 / neo and HepG2 (P <0.05). HepG2 / hB7-1 vaccine stimulated the expression of HLA-I antigen in lymphocytes, and the secretion of IFN-γ and TNF were higher than that of HepG2 / neo and HepG2 groups. The killing rates of LAK cells to HepG2 / hB7-1 cells at E / T = 40: 1 were 2.0 and 2.4 times that of HepG2 / neo and HepG2 cells, respectively. The cytotoxicity of CTL cells to HepG2 / hB7-1 cells was HepG2 / neo and HepG2 2.7 times the killing, with significant differences (P <0.001). Conclusion: The activation of T cells in anti-HCC immunization requires double-signal costimulation. B7-1 molecule can induce in vitro anti-liver cancer immune response.