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通过三种途径得到了稻瘟病菌产生链状着生分生孢子的突变体菌株 ,并对这些突变菌株的DNA指纹图谱、致病性及孢子形成等特性与原菌株分别作了比较研究。用复合位点探针MAGGY和MGR5 86进行的DNA指纹图谱分析结果表明 ,这些突变体确系野生型稻瘟病菌的突变体。虽然这些突变菌株都能产生病斑 ,但其致病性较野生型菌株有明显下降。叶鞘接种结果表明 ,所用突变菌株均能形成附着孢并能穿透寄主细胞壁。突变菌株能形成链状着生的分生孢子 ,多数有两个分隔 ,呈弯膝形或长镰刀状 ,透明或灰橄榄色。突变菌株在病斑上和燕麦片培养基上都能产生链状着生的分生孢子 ,也能产生正常的分生孢子。通过单孢分离后 ,在燕麦片培养基上形成的两种孢子的比例基本稳定 ,表明突变菌株的孢子形成是一种受遗传控制的稳定性状
Three strains were obtained by the pathogenicity of the strain Pyricularia oryzae to produce conidia of Streptomyces strains, and the DNA fingerprinting, pathogenicity and sporulation of these mutant strains were compared with the original strain. DNA fingerprinting analysis using multiplexed site probes MAGGY and MGR5 86 showed that these mutants were indeed mutant of the wild-type Magnaporthe grisea. Although all of these mutant strains can produce spot, their pathogenicity is significantly lower than that of wild type strain. Leaf sheath inoculation results show that all the mutant strains can form attached spores and penetrate the host cell wall. Mutant strains can form conidiospores with a majority of two contiguous, curved knees or long sickles, clear or gray olive. Mutant strains produce both conidia and normal conidia on both the lesion and the oatmeal medium. The ratio of the two spores formed on the oatmeal medium was essentially stable after single spore separation, indicating that the sporulation of the mutant strain is a well-controlled,