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以猪粪为原料,小麦秸秆为调节物质,在小型厌氧发酵装置进行中温(37℃)厌氧发酵试验,研究了外源添加土霉素(OTC)对厌氧发酵过程中累积甲烷产量、脲酶活性和脱氢酶活性的影响以及土霉素的浓度变化情况.依据土霉素的添加量不同,试验共设置4个处理:CK处理,L处理(OTC质量分数60 mg·kg-1),M处理(OTC质量分数100 mg·kg-1),H处理(OTC质量分数140 mg·kg-1).结果表明:厌氧发酵初期,土霉素对甲烷累积产量有一定的促进作用,以后转变为抑制作用.厌氧发酵结束时,同对照相比,L至H处理甲烷累积产量依次减少了8.9%,4.6%,9.4%.厌氧发酵初期,土霉素对脲酶活性有一定的促进作用,随后转变为抑制作用;厌氧发酵前期,不同浓度土霉素对脱氢酶活性均表现为抑制作用,厌氧发酵后期,只有H处理的脱氢酶活性显著小于对照.土霉素含量在第10 d大幅度减少,30 d后土霉素含量基本维持不变.
With pig manure as raw material and wheat straw as regulator, anaerobic fermentation was conducted in a small anaerobic fermentation at medium temperature (37 ℃). The effects of OTC on methane production, Urease activity and dehydrogenase activity as well as the change of oxytetracycline concentration.Under the different amount of oxytetracycline, four treatments were set up: CK treatment, L treatment (OTC 60 mg · kg-1) (OTC mass fraction 100 mg · kg-1) and H treatment (OTC mass fraction 140 mg · kg-1). The results showed that oxytetracycline could promote the methane production in the early stage of anaerobic fermentation, After the end of anaerobic fermentation, compared with the control, L to H cumulative methane production decreased by 8.9%, 4.6%, 9.4% .At the beginning of anaerobic fermentation, oxytetracycline urease activity to a certain extent Promote the role, then change into the inhibition; anaerobic fermentation, different concentrations of oxytetracycline on dehydrogenase activity showed inhibition, late anaerobic fermentation, only the H treatment dehydrogenase activity was significantly less than the control .Oxytetracycline The content decreased significantly on the 10th day and the content of oxytetracycline remained basically unchanged after 30 days.