O 磷酸酰化多肽及类似物对研究和阐明蛋白质可逆磷酸化对生命活动的调节机制具有极其重要的作用 ,本文总结了近年来发展起来的用现代化学方法合成O 磷酸酰化多肽及类似物的有效策略 ,如总体磷酸化法、磷酸化单体法等 ,并概述了常用的磷酸化方法及磷肽类似物的合成方法等 O Phosphorylated polypeptides and their analogues play an important role in the research and elucidation of the regulatory mechanism of reversible phosphorylation of proteins on life activities. In this paper, we summarize recent advances in the synthesis of O-phosphorylated polypeptides and their analogs by modern chemical methods Effective strategies such as total phosphorylation, phosphorylated monomer, etc., and general methods of phosphorylation and synthesis of phosphopeptide analogues